Regression of Melasma with Platelet-Rich Plasma Treatment

Ann Dermatol. 2014 Jun; 26(3): 401-402.
PMCID: PMC4069656
Published online 2014 Jun 12. doi: 10.5021/ad.2014.26.3.401
PMID: 24966645
Regression of Melasma with Platelet-Rich Plasma Treatment
Mutlu Çayırlı, Ercan Çalışkan,Gürol Açıkgöz,Ahmet Hakan Erbil,and Güneş Ertürk
Dermatology Service, Mevki Military Hospital, Ankara, Turkey.
1Department of Dermatology, Gulhane School of Medicine, Ankara, Turkey.
2MD Dermatology Clinic, Istanbul, Turkey.
Corresponding author.
Corresponding author: Mutlu Çayırlı, Mevki Military Hospital, Dermatology Service, Dışkapı- Altındağ, 06290 Ankara, Turkey. Tel:
90-537-620-76-79, Fax: 90-312-311-46-09, [email protected]
Received 2013 Apr 3; Revised 2013 May 11; Accepted 2013 May 29.
Copyright © 2014 The Korean Dermatological Association and The Korean Society for Investigative Dermatology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License
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This article has been cited by other articles in PMC.
Dear Editor:
Platelet-rich plasma (PRP) treatment is performed via the autologous injection of high concentration of
platelets in a small volume of plasma1.
A 27-year-old woman presented at our dermato-cosmetology department for skin rejuvenation, as she
had epidermal hyperpigmentation over the cheeks, perioral region, and forehead for about 5 years. PRP
treatment was initiated and her face was injected with autologous PRP prepared by using RegenKit®
(Regen Lab., Le Montsur-Lausanne, Switzerland). Before the treatment sessions, 8 ml of blood was
collected from the patient into a special tube containing a separation gel and an anticoagulant. The tube
was then centrifuged for 8 minutes at 3,500 rpm, and PRP was obtained from the upper part of the buffy
coat. A 32-G needle was used for superficial microinjections via the mesotherapy technique, and the
injections were administered in to the papillary dermis (1.5~2.0 mm deep). Approximately 1.5 ml of
PRP was injected in to the dermis of the face at each session with 15-day intervals. At the end of the
third session of PRP treatment, >80% reduction in epidermal hyperpigmentation was observed (Fig. 1,
2). We did not provide any other treatment or post treatment care besides prescribing the use of a sun-
screen product. There has been no recurrence of melasma for 6 months now.
Fig. 1
Hyperpigmentation over the cheeks, perioral region, and forehead.
Fig. 2
Significant regression in epidermal hyperpigmentation.
PRP is commonly used in dermatology and plastic surgery, especially for treating chronic wounds,
ulcers, and burns. In recent years, PRP has also started to be used in the field of cosmetology1.
Volumetric filling, skin rejuvenation, acne scars, and alopecia are the main targets of PRP application in
The most important contents of platelets are contained in the α-granules. There are >30 bioactive
substances in these granules2. Some of the bioactive substances present in the α-granules include
platelet-derived growth factor (PDGF), transforming growth factor (TGF)-β1, 2, epidermal growth
factor, and mitogenic growth factors such as platelet-derived angiogenesis factor and fibrinogen3. To
our knowledge, only TGF-β1 has been investigated about its relation with melanogenesis.
Kim et al.investigated the effects of TGF-β1 on melanogenesis by using a spontaneously immortalized
mouse melanocyte cell line, and asserted that TGF-β1 significantly inhibits melanin synthesis in a
concentration-dependent manner. They declared that TGF-β1 decreases melanogenesis via delayed
extracellular signal-regulated kinase activation.
The pigmentary improvement that occurs with PRP treatment may be associated with the increase in
skin volume. PDGF has a significant role in blood vessel formation and in the synthesis of collagen and
components of the extracellular matrix, including hyaluronic acid. Hyaluronic acid has been shown to
increase skin tone and volume, resulting in providing a more ‘glowing skin’5.
Although, it is not possible to reach a definitive conclusion, we consider the regression of melasma after
PRP treatment as an interesting finding. Controlled clinical trials are needed to confirm this preliminary
Go to:
1. Kim DH, Je YJ, Kim CD, Lee YH, Seo YJ, Lee JH, et al. Can platelet-rich plasma be used for skin
rejuvenation? Evaluation of effects of platelet-rich plasma on human dermal fibroblast. Ann Dermatol.
2011;23:424-431. [PMC free article] [PubMed]
2. Eppley BL, Pietrzak WS, Blanton M. Platelet-rich plasma: a review of biology and applications in
plastic surgery. Plast Reconstr Surg. 2006;118:147e-159e. [PubMed]
3. Smyth SS, McEver RP, Weyrich AS, Morrell CN, Hoffman MR, Arepally GM, et al. 2009 Platelet
Colloquium Participants. Platelet functions beyond hemostasis. J Thromb Haemost. 2009;7:1759-1766.
4. Kim DS, Park SH, Park KC. Transforming growth factor-beta1 decreases melanin synthesis via
delayed extracellular signal-regulated kinase activation. Int J Biochem Cell Biol. 2004;36:1482-1491.
5. Papakonstantinou E, Roth M, Karakiulakis G. Hyaluronic acid: a key molecule in skin aging.
Dermatoendocrinol. 2012;4:253-258. [PMC free article] [PubMed]
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